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microanalytical and -synthetical methods

List of microanalytical methods offered in BioSupraMol and of according instruments available from OpenIRIS providers and working groups.

BioSupraMol_200x109_300r_x1.545

Cell and microbiology

Methods:

  • Working with biological, cell, and microbe samples up to risk class 2 (RG2)
  • Working under clean room conditions (product and operator protection)
  • Freeze drying of biological samples
  • (Ultra)-centrifugation of biological samples
  • Flow cytometry
  • Fluorescence and transmitted light microscopy
  • Life-dead assay
  • Virus and Bactera assays
  • Toxicity tests

Devices:

  • Bench Safe2020 ( BA-Unit, SupraFAB)
  • Optima MAX-XP ( BA-Unit, SupraFAB)
  • Christ Lyophylle ( BA-Unit, SupraFAB)
  • Attune NxT ( BA-Unit, SupraFAB)
  • BD Accuri C6 ( BA-Unit, SupraFAB)
  • SPARK multimode ( BA-Unit, SupraFAB)
  • M200 P Infinite Pro ( BA-Unit, SupraFAB)
  • Epoch 2 ( BA-Unit, SupraFAB)
  • Zeiss Axioskop 40 POL ( BA-Unit, AG Haag)
  • Zeiss Axio Observer 7 ApoTome.2 ( BA-Unit, SupraFAB)
  • Zeiss Axio Observer.Z1 (ColorCam) ( BA-Unit, SupraFAB)
  • Zeiss Axio Observer.Z1 (Incubator) ( BA-Unit, SupraFAB)
  • Zeiss Axio Vert.A1 ( BA-Unit, AG Haag)
  • Zeiss PrimoVert ( BA-Unit, SupraFAB)

Protein and nucleic acid analysis

Methods:

  • MST - MicroScale Thermophoresis
  • PCR - Polymerase Chain Reaction
  • SPR - Surface Plasmon Resonance
  • Gel imaging
  • Plate reading (fl, lu, abs)
  • Ultra centrifugation
  • Automated analysis systems
  • Clotting test
  • Expression analysis

Devices:

  • ÄKTA pure 25 ( BA-Unit, SupraFAB)
  • Agarose Gel Equipment ( BA-Unit, SupraFAB)
  • SDS Page and Western Blot Equipment ( BA-Unit, SupraFAB)
  • Chemidoc MP ( BA-Unit, SupraFAB)
  • UV solo TS ( BA-Unit, SupraFAB)
  • STart MAX ( BA-Unit, SupraFAB)
  • Prometheus Panta ( Analytics-Lab, SupraFAB)
  • Tycho NT.6 ( Analytics-Lab, SupraFAB)
  • Optima MAX-XP ( BA-Unit, SupraFAB)
  • Biacore X100 ( BA-Unit, SupraFAB)
  • CFX Opus 96 qPCR ( BA-Unit, SupraFAB)
  • Gradient PCR Cycler ( BA-Unit, SupraFAB)
  • SPARK multimode ( BA-Unit, SupraFAB)
  • M200 P Infinite Pro ( BA-Unit, SupraFAB)
  • Nanodrop 2000c ( BA-Unit, SupraFAB)
  • MST label free ( BA-Unit, SupraFAB)
  • MST Monolith NT.115 ( BA-Unit, SupraFAB)
  • MST Monolith NT.115 Pico ( BA-Unit, SupraFAB)

Microfluidics

Methods:

  • biological data analysis
  • chip design
  • active microfluidic
  • cell response
  • mask fabrication 
  • PDMS chips fabrication
  • video (transmitted light and fluorescence)
  • discrete microfluidics
  • discrete particle synthesis
  • soft-bake lithography
  • encapsulation of cells in microgels

Devices:

  • Harvard PHD ULTRA (4 Stk.) ( BA-Unit, SupraFAB)
  • WS-650Mz-23NPPB spin coater (MF-Unit, SupraFAB)
  • THINKY ARE-250 (MF-Unit, SupraFAB)
  • PICO lp system (MF-Unit, SupraFAB)
  • UV-LED KVB 2 (MF-Unit, SupraFAB)
  • Zeiss Axio Observer.Z1 (Incubator) (BA-Unit, SupraFAB)
  • Zeiss PrimoVert (BA-Unit, SupraFAB)

Surface spectroscopy

Methods:

  • SPR - Surface Plasmon Resonance
  • XPS - X-ray Photoelectron Spectroscopy
  • EPMA - Electron Probe Micro Analysis

Devices:

  • Biacore X100 SPR Spektrometer ( BA-Unit, SupraFAB)
  • Reichert 4SPR ( BA-Unit, SupraFAB)
  • EnviroESCA XPS ( SA-Unit, SupraFAB)
  • JEOL JXA 8200 Superprobe ( Geo-Unit, BSM)

Calorimetry

Methods:

  • Isothermal titration calorimetry (ITC)

Devices:

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