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BioSupraMol

Available methods in BioSupraMol

This page provides quick access to the methods and equipment provided at the department (and beyond) as well as references to the providers. Users who already know exactly what they are looking for can try the methods index below.

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classical analytical methods

Among the classical analytical methods we include elemental analysis and gravimetry, as well as routine spectroscopic measurements from infra red to ultraviolett; nuclear magnetic resonance and mass spectrometry. However, our modern spectroscopes can also be used for highly complex and specialised measurements beyond any routine.

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  • Elementary analysis (EA)

› CHN and CHNS analysis

 

  • Gravimetrie

› Thermo Gravimetric Analysis (TGA)

 

  • Kerneresonanz Spektroskopie (NMR)

› 1H, 19F, 13C, 31P, 29Si, 15N
› DOSY
› HR-MAS
› solid state NMR

 

  • Massenspektrometrie (MS)

› Matrix Assisted Laser Desorption Ionisation (MALDI)
› Time-Of-Flight (TOF)
› GC- and HPLC-MS

 

  • Lichtspektroskopie

› Absorption spectroscopy
› Emission spectroscopy
› IR spectroscopy
› Raman spectroscopy
› Light scattering

 

imaging methods

Among the imaging methods such methods are listed, which result in direct or indirect images of the (macro)molecule or of the molecular or supramolecular architectures. Depending on the desired resolution there are many light or electron optical methods, but also x-ray structural analysis or atomic force microscopy.

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  • Optical microscopy (OM)

› Stimulated Emission Depletion Microscopy (STED)
› Confocal Laser Scanning Microscopy (CLSM)
› Total Internal Reflection Fluorescence (TIRF)
› Stochastic Optical Reconstruction Microscopy (STORM)
› Fluorescence microscopy with structured ilumination
› Life cell imaging

 

  • Electron microscopy (EM)

› Transmission Electron Microscopie (TEM)
› Scanning Transmission Elektron Microscopy (STEM)
› Scanning Elektron Microscopy (SEM)

 

  • X-ray structure analysis (XRD)

› Powder diffraction
› Single cristall diffraction

 

  • Surface imaging

› Atomic Force Microscopy (AFM)
› Scanning Elektron Microscopy (SEM)
› Secondary Ion Mass Spectrometry (SIMS)
› Electron Probe Micro Analysis (EPMA)

 

micro methods

In the group of micro methods we primarily put together bioanalytical and microanalytical and synthetical procedures. The often required biolabs with S2 safety accredition are located at the new SupraFAB research building. Due to the special safety level, appropriate biosafety training is mandatory to use the state-of-the-art devices their.

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  • Cell and microbiology

› S2 certified labs
› sterile operation in biological safety benches
› cell, bacteria, and virus cultivation
› freeze drying and (ultra)centrifugation of bio samples
› Perlocation cytometry
› Fluorescence and transmitted light microskopie

 

  • Protein and nucleic acid analysis

› Purification
› Quality & quantity determination
› Interaction studies
› Thermophoresis

 

  •  Surface spectroscopy

› Surface plasmon resonance spectroscopy (SPR)
› X-ray photoelectron spectroscopy (XPS)

 

  • Micro fluidic (MF)

› Chip design and fabrication
› Discrete microfluidics
› Active microfluidic

 

  • Calorimetry

› Isothermal Titration Calorimetry (ITC)

 

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